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Purpose: This protocol describes how to quantify any type of cellular foci, such as phosphorylated Ser139 on histone variant H2A. Misleading westerns: common quantification mistakes in western blot densitometry and proposed corrective measures. Quantification of foci using Fiji or ImageJ. Journal of Neuroscience Methods, 172(2), 250–254.īutler, T.A.J. This protocol offers an Image J macro/plugin that enable easy quantification of bands on western blots, dot blots, and fluorescent gels etc. The use of total protein stains as loading controls: An alternative to high-abundance singleprotein controls in semiquantitative immunoblotting. Due to the technical knowledge required for densitometry analysis together with the resources availability, standard office scanners are often used for the imaging acquisition of developed Western blot films. Keywords: blotting, statistical analysis, non-parametric, densitometryĪldridge, G.M. 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